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THUNDER™ Total 4EBP1 TR-FRET Cell Signaling Assay Kit
品牌：Cayman
货号：500226-2400 wells 规格：2400 wells 目录价：￥79472
货号：500226-480 wells 规格：480 wells 目录价：￥18736
货号：500226-4800 wells 规格：4800 wells 目录价：￥98784
货号：500226-96 wells 规格：96 wells 目录价：￥5968
货号：500226-9600 wells 规格：9600 wells 目录价：￥122896
产品详情
产品名称:
THUNDER™ Total 4EBP1 TR-FRET Cell Signaling Assay Kit
别名:
eIF4E-binding Protein 1|PHAS-I|Phosphorylated Heat- and Acid-Stable Protein Regulated by Insulin 1
产品描述:
The Total 4EBP1 assay kit is a homogeneous time-resolved Förster resonance energy transfer (TR-FRET) sandwich immunoassay. The THUNDER™ Cell Signaling assay workflow consists of 3 steps. Following cell treatment, cells are first lysed with the specific Lysis Buffer provided in the kit. Then Total 4EBP1 in the cell lysates is detected with a pair of fluorophore-labeled antibodies in a simple "add-incubate-measure" format (single-step reagent addition; no wash steps). One antibody is labeled with a donor fluorophore (Europium chelate; Eu-Ab1) and the second with a far_x0002_red acceptor fluorophore (FR-Ab2). The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm. The signal at 665 nm is proportional to the concentration of Total 4EBP1 in the cell lysate. Data can be expressed as either the signal at 665 nm or the 665 nm/615 nm ratio.
保存温度:
-80
运输温度:
-80
有效期:
180
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