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折扣/货期| 货号：SBB-KF0030-KI01 | 规格：1 Kit | 目录价：￥14168 |
产品详情
产品名称:
Mdm2 E3 Ligase TR-FRET Kit
别名:
HDM2
产品描述:
South Bay Bio’s MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
靶标/特异性:
Readout: Endpoint/Kinetic
Label or Dye: Europium-Cryptate & Cy5
Detection Method: TR-FRET
Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x.
Excitation/Emission (nm): 304/620 & 304/665
保存温度:
-80°C
运输温度:
Dry Ice
产地:
瑞士
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