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BRD4 bromodomain 1 TR-FRET Assay Kit
品牌：Cayman
货号：600520-1920 wells 规格：1920 wells 目录价：￥30086
货号：600520-384 wells 规格：384 wells 目录价：￥8036
货号：600520-9600 wells 规格：9600 wells 目录价：￥134624
产品详情
产品名称:
BRD4 bromodomain 1 TR-FRET Assay Kit
别名:
Bromodomain containing protein 4|HUNK1|MCAP
产品描述:
Cayman’s BRD4 bromodomain 1 TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of BRD4 bromodomain 1 (human amino acids 49-170) directly labeled with a europium (Eu3+) chelate. A biotinylated peptide containing acetylated lysines (BRD-Eu3+) serves as the ligand for BRD4 bromodomain 1. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-avidin conjugate resulting in a loss of TR-FRET signal. The BRD4 bromodomain 1 TR-FRET Assay Kit is robust (Z' = >0.85) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.
保存温度:
-80
运输温度:
-80
有效期:
365
来源:
Animal/Bovine
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