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    Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated
    品牌：EpiCypher
    货号：16-0384 规格：50 µg 目录价：￥6900
    产品详情
    产品名称:
    Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated
    产品描述:
    Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*); H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains butyryl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. *H3K27bu has a Cys to Ala substitution at position 110.
    保存温度:
    Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
    运输温度:
    Dry ice.
    产品形式:
    H3K27bu dNuc (27.4 µg protein weight, 50 µg DNA + protein) in 53.8 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 4.65 μM. MW = 199,903.6 Da
    产地:
    美国
    生产商:
    EpiCypher
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