货号:16-0382 | 规格:50 µg | 目录价:¥6900 |
产品详情
产品名称:
Nucleosome, Recombinant Human, H3R17me1 dNuc, Biotinylated
产品描述:
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains monomethylated arginine at position 17. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. *H3R17me1 has a Cys to Ala substitution at position 110.
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
H3R17me1 dNuc (27.3 µg protein weight, 50 µg DNA + protein) in 62.8 µL of 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 3.98 μM. MW = 199,804.06 Da.
产地:
美国
生产商:
EpiCypher
上一个:Nucleosome, Recombinant Human, H2BK20ac dNuc, Biotinylated
下一个:Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated
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