H3T6phos Recombinant Nuclesosome, Biotinylated-突变/修饰核小体-深圳九游会j9网站首页有限公司

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H3T6phos Recombinant Nuclesosome, Biotinylated

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：16-0405

规格：50 µg

目录价：￥6900

产品详情

产品名称:

H3T6phos Recombinant Nuclesosome, Biotinylated

产品描述:

Histone phosphorylation is a post-translational modification (PTM) wherein a phosphate group is added to a histone protein, predominantly occurring on serine, threonine, and tyrosine residues. In combination with other PTMs, histone phosphorylation constitutes the “histone code,” acting as a language read by proteins to regulate chromatin structure and gene expression. Histone phosphorylation is involved in chromatin remodeling and compaction associated with diverse cellular processes, including DNA damage repair, transcription regulation, cell division, and apoptosis [1]. Recombinant mononucleosomes containing phosphorylated histones can be used to study the biological functions of histone phosphorylation. H3T6phos (histone H3 threonine 6 phosphorylation) Recombinant Nucleosome, Biotinylated consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [2], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. H3T6phos nucleosome contains phosphorylated threonine at position 6 on histone H3.2 and a Cys to Ala substitution at position 110. H3T6phos is associated with transcription regulation via H3 methylation, preventing LSD1 demethylation of H3K4 during androgen receptor-dependent gene activation [1].

宿主来源:

Human

表达系统:

E. coli & synthetic DNA

保存温度:

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

运输温度:

Dry ice.

产品形式:

1.1 mg/mL mononucleosome in 45.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.2 µg protein, 50 µg DNA + protein).

产地:

美国