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Mononucleosomes, Recombinant Human, Desthiobiotinylated
品牌：EpiCypher
货号：16-0024 规格：50 µg 目录价：￥5700
产品详情
产品名称:
Mononucleosomes, Recombinant Human, Desthiobiotinylated
产品描述:
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1, and H4 Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (Kd = 10-11) for avidin-family proteins than does biotin (Kd = 10-15).
保存温度:
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
Purified recombinant mononucleosomes (50 µg total mass, 27.3 µg protein + 23.7 µg DNA in 42.5 µL) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. Concentration of nucleosomes is 5.89 µM. Nucleosome molecular weight = 199,827.86 Da.
产地:
美国
生产商:
EpiCypher
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