货号:16-0018 | 规格:50 µg | 目录价:¥5700 |
产品详情
产品名称:
Mononucleosomes (H4ΔN15), Human Recombinant Biotinylated
产品描述:
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4 (ΔN15). Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H4 beginning with lysine 16 (amino acids 1-15 are deleted), wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
保存温度:
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
Purified recombinant mononucleosomes (50 µg total mass, 27.4 µg protein) in 53 µL 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. Concentration of nucleosomes is 4.78 µM. Nucleosome molecular weight = 197,323.28 Da.
产地:
美国
生产商:
EpiCypher
上一个:Mononucleosomes (H3.3 ΔN32), Human Recombinant
下一个:Mononucleosomes (H3.1ΔN32), Human Recombinant, Biotinylated
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