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Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated
品牌：EpiCypher
货号：16-0389 规格：50 µg 目录价：￥6900
产品详情
产品名称:
Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated
产品描述:
EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one or more histone post translational modifications (PTMs). Nucleosomes, the basic repeating unit of chromatin, are the natural targets of chromatin readers and modifying enzymes, providing a physiologically relevant substrate for characterization of epigenetic proteins. dNucs consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.3, and H4). The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3.3S31phos dNuc contains phosphoserine at position 31 on histone H3.3, which has shown to be a negative regulator of KDM4B binding, and thereby preserving H3K9me3 marks [2]. The DNA in this nucleosome contains a 5’biotin-TEG group.
宿主来源:
Human
表达系统:
E. coli & synthetic DNA
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
运输温度:
Dry ice.
产品形式:
0.78 mg/mL mononucleosome in 64.1 uL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.4 µg protein, 50 µg DNA + protein)
产地:
美国
生产商:
EpiCypher
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