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Nucleosome, Recombinant Human, H2AK119ub1 dNuc, Biotinylated
品牌:EpiCypher
货号:16-0395 规格:25 µg 目录价:¥6300
产品详情
产品名称:
Nucleosome, Recombinant Human, H2AK119ub1 dNuc, Biotinylated
产品描述:
H2AK119ub1 designer mononucleosomes (dNucs) consist of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The dominant form of ubiquitinated histones are monoubiquitinated H2A and H2B. Ubiquitination of H2A is usually correlated with transcriptional repression, while ubiquitination of H2B is more frequently correlated with transcriptional activation. Histone ubiquitination can be induced by DNA damage, therefore playing an important role in DNA damage response. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA in this nucleosome contains a 5’ biotin-TEG group.
宿主来源:
Human
表达系统:
E. coli & synthetic DNA
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
运输温度:
Dry ice.
产品形式:
0.98 mg/mL H2AK119ub1 dNuc in 25.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. (14.4 µg protein, 25 µg DNA + protein)
产地:
美国
生产商:
EpiCypher
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