Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A-突变/修饰核小体-ELISA_抗体_蛋白_核苷酸

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Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：16-1029

规格：50 µg

目录价：￥5700

产品详情

产品名称:

Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A

产品描述:

Recombinant mononucleosomes consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. Histone H2A contains a glutamate-to-alanine (E-to-A) substitution at position 61 (H2AE61A). H2AE61A is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE61 resides in the alpha2 helix and forms a key salt bridge with H4K16. H2AE61 mediates chromatin binding with factors such as LANA, RCC1, IL-33, SIR3, and HMGN2 [2]. H2AE61A disrupts chromatin remodeling by the ISWI remodeler SMARCA5/SNF2h [4].

宿主来源:

Human

表达系统:

E. coli & synthetic DNA

保存温度:

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

运输温度:

Dry ice.

产品形式:

0.84 mg/mL mononucleosome in 59.8 uL 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. (27.3 µg protein, 50 µg DNA + protein).

产地:

美国