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NAPRTase (human) (rec.) (His)
品牌：Adipogen
货号：AG-40T-0105-C050 规格：50 ug 目录价：￥4680
产品详情
产品名称:
NAPRTase (human) (rec.) (His)
别名:
Nicotinate Phosphoribosyltransferase; FHA-HIT-interacting Protein; FHIP; EC 2.4.2.11
产品描述:
Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosesynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosesynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
宿主来源:
E. coli
产品浓度:
0.5mg/ml (Lot specific)
保存温度:
-80°C
运输温度:
DRY ICE
预期分子量:
~58.0kDa
产品形式:
liquid
存储溶液:
Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.
有效期:
Stable for at least 6 months after receipt when stored at -80°C.
产地:
瑞士
纯度:
>98% (SDS-PAGE)
生物活性:
0.043µmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 µM PRPP, 100 µM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 µM, 25°C, spectrophotometrical monitoring.
内毒素水平:
N/A
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