LIGHT/CD258 Extracellular Domain (human, recombinant)

HVEML|TNFSF14|TR2|Tumor Necrosis Factor Ligand Superfamily Member 14

LIGHT, also known as CD258, is a type II transmembrane glycoprotein encoded by TNFSF14 in humans.{61299} Alternative splicing of the TNFSF14 pre-mRNA produces a cytosolic isoform, as well as a membrane-bound isoform, which can be proteolytically cleaved by metalloproteinases to produce a soluble form.{62077,62078} It exists as a homotrimer and is constitutively expressed on immature dendritic cells and transiently expressed on activated T cells.{62078,61299} LIGHT is also expressed by other hematopoietic cells, including natural killer (NK) cells, monocytes, and granulocytes.{61300,62077} It binds to herpes virus entry mediator (HVEM) (Item No. 31840), also known as CD270, as well as the lymphotoxin β receptor (LTβR), where it acts as a costimulatory molecule for T, B, and dendritic cells by promoting cytokine secretion, leading to cell differentiation and activation.{62077,61301} Tnfsf14-/- mice have increased mortality in a model of experimental autoimmune encephalitis (EAE), whereas Tnfsf14-/- mice have reduced inflammation in a model of graft versus host disease (GVHD), indicating additional roles in immune regulation.{62079,61299} Cayman’s LIGHT/CD258 Extracellular Domain (human, recombinant) protein can be used for enzyme activity assays. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of LIGHT/CD258 (amino acids 74-240) fused to human IgG1 Fc at its N-terminus, consists of 404 amino acids, and has a calculated molecular weight of 44.9 kDa.

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥90% as determined by SDS-PAGE