Leptin (human, recombinant)

LEPD|Obesity Factor|OBS

Leptin is a peptide hormone that regulates energy homeostasis, growth, and reproduction, as well as immune function.{60447} It is secreted by adipocytes and regulated by nutritional status, where fasting reduces leptin levels and feeding increases leptin levels, as well as in a circadian manner, with serum levels peaking at night.{60449,60450} Circulating leptin can bind leptin receptors expressed by peripheral immune cells, such as macrophages, natural (NK) cells, and B and T cells, or be transported across the blood brain barrier, where it binds to neuronally expressed leptin receptors.{60447} Binding of leptin to the leptin receptor induces the recruitment and phosphorylation of JAK2, leading to activation of a variety of intracellular signaling cascades, including MAPK-, STAT-, PI3K-, and AMPK-mediated pathways.{60449} Leptin-dependent signaling pathways promote energy expenditure, growth, and reproductive functions, reduce food intake and the production of adrenal corticosteroids, and enhance innate and adaptive immune responses.{60447} Leptin-deficient ob/ob mice exhibit severe obesity, hyperlipidemia, hyperglycemia, and hyperinsulinemia and are a commonly used model of obesity and type 2 diabetes.{60451} Serum leptin levels are increased in individuals with a variety of conditions, including obesity or insulin resistance.{60452} Cayman's Leptin (human, recombinant) protein can be used for binding assays. The protein was synthesized from a DNA sequence encoding the mature form of human leptin (Val22-Cys167) with an N-terminal translation-initiating methionine (Met1). The expressed protein consists of 147 amino acids, has a calculated molecular weight of 16 kDa, and a predicted N-terminus of Met1.

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥98% estimated by SDS-PAGE