Citrullinated Interleukin-6 (human, recombinant)

B Cell Stimulatory Factor 2|BSF-2|CDF|CTL Differentiation Factor|HGF|Hybridoma Growth Factor|IFN-β2|IL-6|Interferon-β2

Interleukin-6 (IL-6) is a cytokine encoded by the IL6 gene in humans with roles in both the initiation and resolution of inflammatory responses.{46747,46748} IL-6 is produced in response to an inflammatory event, induces recruitment of neutrophils to the site of inflammation, and signals through the membrane-bound IL-6 receptor (IL-6R) to upregulate the synthesis of acute phase proteins, lipolysis in the liver, and induced differentiation of T and B cells. Following initiation of this classical signaling pathway, a soluble form of IL-6R (sIL-6R) is released from neutrophils to promote recruitment of monocytes and macrophages, induction of fever via IL-6 action in the hypothalamus, and production of anti-inflammatory M2 macrophages to initiate tissue repair. Production of IL-6 is dysregulated in various chronic inflammatory diseases, including rheumatoid arthritis, Castleman disease, polymyalgia rheumatica, and giant cell arteritis.{46749} It also induces tumor growth and metastasis via activation of JAK/STAT signaling.{46750} Recombinant human IL-6 can be citrullinated at arginine residues by protein arginine deiminase 4 (PAD4) in vitro.{57044} Citrullinated IL-6 and anti-citrullinated peptide antibodies (ACPAs) reactive with citrullinated IL-6 have been found in synovial fluid and sera, respectively, from patients with rheumatoid arthritis. This product contains purified IL-6 (human, recombinant) (Item No. 30174) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography.

-80

-80

50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 10% glycerol, 2 mM β-mercaptoethanol

365

≥85% estimated by SDS-PAGE