TNF配体多聚蛋白——更高的活性，更低的内毒素
反向信号传递现象正逐渐受到人们的关注。简言之 ,即可溶性受体或膜表面受体与表达在细胞膜表面的相应配体结合后 ,传递信号至表达配体的细胞内 ,并引发相应的生物学效应。目前 ,对于这一热点的研究多集中于ＴＮＦ配体超家族成员。Adipogen提供多种TNF配体多聚蛋白，这些蛋白均具有高活性，低内毒素的特点。
TNF Ligands Multimeric Proteins
Higher Activity – Lower Endotoxin
AdipoGen® Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.
| Endogenous TNF superfamily ligands are either active as membrane- form (e.g. FasL, TRAIL, CD40L, OX40L) or are secreted and activated through oligomerization by the binding of pro-teoglycans at the surface of cells (e.g. APRIL).
To mimic endogenous TNF ligands activity, the oligomerization of recombinant TNF ligands can be triggered:
• by fusing the TNF superfamily ligands, soluble form, to the collagen domain of the protein ACRP30 (which itself has no functional activity) to form a hexameric structure and therefore creating “Multimeric Proteins”, or
• by adding a cross-linking antibody called “TNF Ligands Enhancer” (Prod. No. AG-35B-0001). |
MultimericFasL. [MegaFasL.]
FasL (human) (multimeric) (rec.)
AG-40B-0130 10 µg | 3 x 10 µg
MultimericFasL™ very effectively simulates the natural membrane-assisted aggregation of FasL in vivo.
Source: HEK 293 cells.
Sequence: Human FasL (aa 139-281) is fused at the
N-terminus to mouse ACRP30headless
(aa 18-111) and a FLAG®-tag.
Specificity: Binds to human and mouse Fas.
Biological Activity: Induces apoptosis of human Jurkat
T cells at a concentration of<1ng/ml.
Endotoxin Content: <0.01EU/μg purified protein (LAL test; Lonza).
LITerature references: Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) • A Fas agonist induces high levels of apoptosis in haematological malignancies: P. Greaney, et al.; Leuk. Res. 30, 415 (2006)
Figure: Oligomerisation of FasL (human) efficiently triggers Jurkat cell death.
Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (AG-40B-0130), Fc (human):FasL (human) (rec.) (AG-40B-0132), FasL (human) (rec.) (AG-40B-0001) or FasL (human) (rec.) + Enhancer (AG-44B- 0001) (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric), Fc (human):FasL (human) and FasL (human) + Enhancer) kill Jurkat cells at IC50 <0.2ng/ml.
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• MultiPacks for additional big savings !
• Bulk Quantities for in vivo cancer immunotherapy and vaccination studies in mice !
MultimericCD40L™
CD40L (human) (multimeric) (rec.)
AG-40B-0010-C010 10 µg | 3 x 10 µg
• MultimericCD40L™ very effectively mimics the natural membrane-assisted aggregation of CD40L. It is the most potent alternative to activate CD40. No enhancer is required.
• MultimericCD40L™ is a potent B cell activator.
LITerature reference: IgG subclass switch capacity is low in switched and in IgM-
only, but high in IgD+IgM+, post-germinal center (CD27+) human B cells: C. Werner-Favre, et al.; Eur. J. Immunol. 31, 243 (2001)
CD 40L (mouse) (multimeric) (rec.) AG-40B-0020 10 μg | 3 x 10 μg |
CD 40L (rat) (multimeric) (rec.) AG-40B-0107 10 μg | 3 x 10 μg |
Bulk available for in vivo studies!
Full Panel of MultimericLigands™
APRIL (human) (multimeric) (rec.) AG-40B-0017 10 μg | 3 x 10 μg |
APRIL (human) (H98) (multimeric) (rec.) AG-40B-0088 10 μg | 3 x 10 μg Same activity as AG-40B-0017. Does not bind proteoglycans. |
APRIL (mouse) (multimeric) (rec.) AG-40B-0089 10 μg | 3 x 10 μg |
APRIL (mouse) (H98) (multimeric) (rec.) AG-40B-0035 10 μg | 3 x 10 μg |
OX40L (mouse) (multimeric) (rec.) AG-40B-0029 10 μg |
TNF-α (human) (multimeric) (rec.) AG-40B-0019 10 μg | 3 x 10 μg |
TNF-α (mouse) (multimeric) (rec.) AG-40B-0021 10 μg | 3 x 10 μg |
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