Figure   1: CTCF peaks in CUT&RUN

CUT&RUN   was performed as described above. Peaks were called with MACS2. Heatmaps show   CTCF peaks relative to IgG negative control antibody in aligned rows ranked   by intensity (top to bottom) and colored such that red indicates high   localized enrichment and blue denotes background signal. CTCF showed expected   enrichment around the TSS. Despite some observable background in the IgG   control, differential enrichment with the target antibody is as expected.

Figure 2:   CTCF CUT&RUN representative browser tracks

CUT&RUN   was performed as described above. Gene browser shots were generated using the   Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci   are shown.

Figure 3:   Immunohistochemistry data

FFPE   sections of human colon carcinoma (left) and mouse spleen (right) using CTCF   antibody at a dilution of 1:1000.

Figure 4: Immunocytochemistry   data

FFPE section   of human MCF-7 cells examined using CTCF antibody at a dilution of 1:1,000.

Figure 5:   Immunoprecipitation data

EpiCypher   CTCF antibody (6 μg/mg lysate) was used to immunoprecipitate whole cell   lysates (1 mg, 20% of IP loaded) isolated from HEK293T cells. A negative   control IgG antibody and positive control antibodies (Bethyl Laboratories)   were also used to demonstrate specificity of the IP. All CTCF antibodies   target the same CTCF epitope, however, A300-534A-6 is hosted in rabbit. For   blotting immunoprecipitates, EpiCypher CTCF antibody was used at a 1:1,000   dilution.

Figure 6:   Western blot data

Western   analysis of CTCF in whole cell extracts from HEK293T, MCF-7, Jurkat, Hep-G2,   and A-549 cells. Fifty micrograms of lysate was resolved via SDS-PAGE and   detected with a 1:1,000 dilution of CTCF antibody.

13-2014

CTCF CUTANA™ CUT&RUN Antibody

100 µL