4-1BB Ligand Extracellular Domain (human, recombinant)

4-1BBL|CD137 Ligand|CD137L|TNFSF9

TNF ligand superfamily member 9 (4-1BB ligand) is a type II transmembrane glycoprotein and a member of the TNF superfamily.{54191,54192} It is composed of an N-terminal cytoplasmic region, a transmembrane domain, and a C-terminal extracellular tail region that contains a TNF homology domain.{54192,54193} 4-1BB ligand production is induced in antigen-presenting cells (APCs), such as dendritic cells, B cells, and macrophages, by CD40 and toll-like receptor (TLR) activation.{54192} It forms homotrimers and binds to its receptor, 4-1BB, on antigen-primed T cells, which induces NF-κB and MAPK signaling through TNF receptor-associated factors (TRAFs) and, when the T cell is co-stimulated by other factors, induces PI3K-Akt signaling.{54191,54192} This action promotes the proliferation of T cells, production of cytokines, and cell survival.{54192} Soluble forms of 4-1BB ligand, consisting of only the extracellular domain, also form trimers that bind to its receptor but do not induce signaling.{54194} Mice deficient for 4-1BB ligand, Tnfsf9, have impairments in the formation of CD8+ T cell memory cells and in clearing pathogens.{54192,54197} Tnfsf9 knockout mice also develop B cell lymphomas before 12 months of age.{54195} 4-1BB ligand antibodies induce T regulatory cell expansion and prevent allogeneic graft rejection of pancreatic islets in mice.{54196} Intratumoral adenovirus-mediated gene transfer of Tnfsf9 induces tumor regression in a mouse syngeneic model of breast cancer.{54195} The protein levels of 4-1BB ligand are elevated in more than 40% of patients with acute myeloid leukemia (AML) or non-Hodgkin’s lymphoma but the expression of TNFSF9 is either elevated or reduced in cancer patients depending on the type of cancer. Cayman’s 4-1BB Ligand Extracellular Domain (human, recombinant) protein can be used for ELISA and binding assay applications. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of 4-1BB ligand (amino acids 71-254) fused to human IgG1 Fc at the N-terminus, consists of 444 amino acids and has a calculated molecular weight of 47.9 kDa.

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥85% estimated by SDS-PAGE