Enzo Life Sciences的ROS-ID® Total ROS/Superoxide detection kit包括两种荧光染料作为主要成分：非荧光、可渗透细胞的氧化应激检测试剂（绿色，Ex/Em 490/525 nm）与多种活性物质（过氧化氢、过氧亚硝酸根和羟基）直接反应，产生绿色荧光产物。超氧化物检测试剂（橙色，Ex/Em 550/620 nm）是一种可渗透细胞的探针，可与超氧化物特异性反应，产生橙色荧光产物。试剂盒还提供Pycyanin（ROS诱导剂）和NAC（ROS抑制剂）。
产品特点
● 用于检测活细胞中超氧化物的产生
● 与细胞培养基的主要成分（酚红，FBS和BSA）兼容
● 已通过显微镜、流式细胞仪和微孔板等应用验证
实验示例
图1. 在加入了ROS/超氧化物检测试剂并用绿脓菌素处理的 HeLa 细胞中，通过荧光显微镜对活性氧的形成进行了分析。在绿色通道中监测一般氧化应激水平，而在橙色通道中检测超氧化物的产生。用 NAC（一种通用的 ROS 抑制剂）预处理可防止 ROS 的形成。
图2. 使用ROS-ID® Total ROS/Superoxide detection kit和ROS清除剂/抑制剂分析用抗霉素A（AMA，特异性超氧化物诱导剂）、叔丁基-过氧化物（TBHP，特异性过氧化物诱导剂）和绿脓菌素（通用ROS诱导剂）处理的HeLa细胞中ROS的产生。使用U-2 OS和CHO K1细胞获得了类似的结果。
图3. 通过流式细胞术分析Hela细胞中ROS的形成。数据以绿脓菌素（ROS/SO诱导剂）、TBHP（ROS 诱导剂）和 AMA（超氧化物诱导剂）处理后的阳性百分比表示。绿色柱表示ROS 阳性率%，橙色柱表示超氧化物阳性率%。
产品信息
产品货号 | ENZ-51010 |
产品名称 | ROS-ID® Total ROS/Superoxide detection kit |
别名 | Reactive oxygen species / Superoxide |
规格 | 1*1Kit (200 fluorescence microscopy assays or 50 flow cytometry assays or 2x96 microplate assays) |
使用/稳定性 | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
短期保存 | -20°C |
长期保存 | -80°C |
试剂盒组分 | Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 µmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack |
应用 | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
部分产品引用文献
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2. Antioxidant effect of nicotinamide mononucleotide in tendinopathy: K. Yamaura, et al.; BMC Musculoskelet. Disord. 23, 249 (2022)
3. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures: I. Jurickova, et al.; Inflamm. Bowel Dis. 28, 988 (2022)
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7. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia: T. Yoshikawa, et al.; BMC Musculoskelet. Disord. 23, 563 (2022)
8. Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy: P.L. Wei, et al.; Int. J. Med. Sci. 18, 3452 (2021)
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10. Dihydroisotanshinone I induced ferroptosis and apoptosis of lung cancer cells: C.Y. Wu, et al.; Biomed. Pharmacother. 139, 111585 (2021)
11. Incense smoke-induced oxidative stress disrupts tight junctions and bronchial epithelial barrier integrity and induces airway hyperresponsiveness in mouse lungs: N. Yamamoto, et al.; Sci. Rep. 11, 7222 (2021)
12. Nano-encapsulation of hydroxytyrosol into formulated nanogels improves therapeutic effects against hepatic steatosis: An in vitro study: E. Mauri, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 124, 112080 (2021)
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