Enzo Life Sciences的ROS-ID® Superoxide detection kit可利用荧光显微镜和/或流式细胞仪直接实时监测活细胞中超氧化物的产生。该试剂盒的一个主要成分，超氧化物检测试剂（橙色），是一种细胞渗透性探针，可与超氧化物发生特异性反应，产生橙色荧光产物。荧光探针对活性过氧化物、羟基、过氧亚硝酸盐、氯或溴相对不敏感，因此该试剂盒不能检测这些分析物。染色后，可使用配备有标准橙色（如550/620 nm）滤光片的宽场显微镜进行观察，或使用配备有蓝色（488 nm）激光的流式细胞仪进行分析。
超氧化物检测试剂盒包含足够的试剂，可使用活细胞（贴壁或悬浮）进行至少200次显微镜分析或50次流式细胞术分析。
产品特点
可通过荧光显微镜或流式细胞术直接监测活细胞中超氧化物的整体水平
高灵敏度、特异性和准确性，可用于活细胞研究
与组织培养基的主要成分（酚红、FBS和BSA）兼容
试剂盒内包括整套所需试剂，包括ROS诱导剂和清除剂
产品信息
产品货号 | ENZ-51012 |
产品名称 | ROS-ID® Superoxide detection kit |
规格 | 1*1Kit |
短期保存 | -20°C |
长期保存 | -80°C |
试剂盒组分 | Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 µmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack |
应用 | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
部分产品引用文献
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2. A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration: K. Bijnens, et al.; Biomolecules 11, 714 (2021)
3. An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination: Y. Wang, et al.; Nat. Commun. 12, 4964 (2021)
4. Assay for advanced glycation end products generating intracellular oxidative stress through binding to its receptor: T. Kobori, et al.; Anal. Biochem. 611, 114018 (2020)
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6. Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy: B. Montico, et al.; Cell Death Dis. 11, 392 (2020)
7. PCSK9 expression in the ischemic heart and its relationship to infarct size, cardiac function and development of autophagy: Z. Ding, et al.; Cardiovasc. Res. 114, 1738 (2018)
8. SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes: K.R. Aseer, et al.; Free Radic. Biol. Med. 108, 874 (2017)
9. Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression: L. Pasterk, et al.; Sci. Rep. 6, 22104 (2016)
10. TRPC6 channel activation promotes neonatal glomerular mesangial cell apoptosis via calcineurin/NFAT and FasL/Fas signaling pathways: H. Soni, et al.; Sci. Rep. 6, 29041 (2016)
11. Hemodynamic Shear Stress via ROS Modulates PCSK9 Expression in Human Vascular Endothelial and Smooth Muscle Cells and Along the Mouse Aorta: Z. Ding, et al.; Antioxid. Redox Signal. 22, 760 (2015)
12. Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells: N. Shin, et al.; Biochem. Biophys. Res. Commun. 459, 699 (2015)
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