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Total Collagen/Hydroxyproline Assay Kit
品牌：Cayman
货号：702440 规格：96 wells 目录价：￥5698
产品详情
产品名称:
Total Collagen/Hydroxyproline Assay Kit
产品描述:
Cayman's Total Collagen/Hydroxyproline Assay Kit provides colorimetric-based methods for measuring collagen/hydroxyproline in tissue, serum, and urine. In this assay, collagen is hydrolyzed to individual amino acids. Hydroxyproline, found almost exclusively in collagen, makes up 11.3% (Col I) -15% (Col III) by mass of the amino acid composition.6 Hydroxyproline is oxidized to a pyrrole intermediate, which reacts with Ehrlich's reagent to create a chromophore with peak absorbance at 560 nm. Both hydroxyproline and collagen standards are included. It is only necessary to use the standard that best fits the needs of your assay. In studies where hydroxyproline measurement is relevant (i.e., collagen metabolism or biosesynthesis) the hydroxyproline standards may be used. The hydroxyproline standards may also be used to calculate collagen levels in appropriate samples by assuming hydroxyproline makes up 11-15% amino acid content of collagen by mass. For a direct measurement of collagen, the collagen standard may be used. Collagen standard will be hydrolyzed along with the samples to give hydroxyproline. The range of this assay is 0.02-10 µg for collagen and 0.006-3 µg for hydroxyproline with a lower limit of quantification (LLOQ) of 0.5 and 0.047 µg for collagen and hydroxyproline, respectively.
保存温度:
-20
运输温度:
-20
有效期:
365
来源:
Animal/Rat
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