SARS-CoV/SARS-CoV-2 Spike Glycoprotein RBD Chimeric Monoclonal Antibody (Clone D001)

SARS-CoV/SARS-CoV-2 Spike RBD|SARS-CoV/SARS-CoV-2 Spike Receptor Binding Domain|SARS-CoV/SARS-CoV-2 Surface Glycoprotein RBD|SARS-CoV/SARS-CoV-2 Surface Glycoprotein Receptor Binding Domain|Severe Acute Respiratory Syndrome Coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 Spike Glycoprotein Receptor Binding Domain

Severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein, also known as the surface glycoprotein, is a viral structural protein encoded by the S gene in SARS-CoV RNA that contains the receptor binding domain (RBD).{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} SARS-CoV spike glycoprotein is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is composed of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46768,46767,49525,49561} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman's SARS-CoV/SARS-CoV-2 Spike Glycoprotein RBD Chimeric Monoclonal Antibody (Clone D001) is composed of human IgG1κ constant domains and variable regions from a mouse immunized with purified recombinant SARS-CoV spike glycoprotein RBD. It can be used for ELISA, flow cytometry (FC), immunocytochemistry (ICC), and immunofluorescence (IF) applications, as well as microneutralization assays.

Chimeric Monoclonal Antibody

ELISA, FC, ICC, IF, MN

Human IgG1κ

Recombinant C-terminal His-tagged SARS-CoV spike glycoprotein RBD

D001

-80

-80

50 or 100 µl of protein A-affinity purified monoclonal antibody

365