SARS-CoV-2 Spike Glycoprotein S1 Subunit (recombinant)

2019-nCoV Surface Glycoprotein S1 Subunit|COVID-19 Surface Glycoprotein S1 Subunit|SARS-CoV-2 Surface Glycoprotein S1 Subunit|Severe Acute Respiratory Syndrome Coronavirus 2 Spike Glycoprotein S1 Subunit

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and the causative agent of COVID-19.{53462,53459,53461,55257,55258} The SARS-CoV-2 spike glycoprotein, also known as the surface glycoprotein, is located on the outer envelope of the virion.{53462} It is composed of an S1 and S2 subunit divided by a furin S-cleavage site not found in other SARS-CoVs.{46767,49561} The S1 subunit contains the receptor-binding domain (RBD), which binds to the carboxypeptidase angiotensin-converting enzyme 2 (ACE2), and the S1 and S2 subunits are cleaved by the protease TMPRSS2 to facilitate viral fusion with the host cell membrane.{49542,49536,49558} The SARS-CoV-2 spike glycoprotein S1 subunit induces inflammatory gene expression in the frontal cortex, hippocampus, and hypothalamus, as well as activates toll-like receptor 2 (TLR2) and TLR4 signaling and increases social avoidance in the juvenile social exploration test in rats.{65535} Cayman's SARS-CoV-2 Spike Glycoprotein S1 Subunit (recombinant) can be used for ELISA. This protein is a disulfide-linked homodimer. The reduced monomer, composed of the SARS-CoV-2 spike glycoprotein S1 subunit (amino acids 16-685) fused to mouse IgG1 Fc at its C-terminus, consists of 904 amino acids and has a calculated molecular weight of 101.4 kDa. As a result of glycosylation, the monomer migrates at approximately 116 kDa by SDS-PAGE under reducing conditions.

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Lyophilized from sterile PBS, pH 7.4

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≥90% estimated by SDS-PAGE