PuroSPIN-96™ Fibrous Tissue RNA Purification Plus Kit, 384 Preps

The PuroSPIN-96™ Fibrous Tissue RNA Purification Kit is designed to purify total RNA from fibrous animal tissue, such as skin, skeletal muscle, lung, or heart. It greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acids binding to 96-well silica membrane purification plates. In comparison to our standard PuroSPIN-96™ Fibrous Tissue RNA Purification Kit, the Plus kit adds an additional filtration step that removes the insoluble and non-digested fraction as well as insuring elimination of genomic DNA from the lysate before loading it onto the 96-well silica purification plate. This prevents contaminating materials from binding to and blocking parts of the silica membrane, leading to an increased RNA yield and higher purity of the final product. This is particularly benficial when workign with large samples that contain a lot of starting material that needs to be digested. This kit includes an on-column DNase I digestion step to remove any contaminating DNA. This kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN™ 96 Fibrous Tissue RNA Purification Kit can be used for a range of downstream applications such as reverse-transcription qPCR, Northern blotting, or microarray analysis. For purification of RNA from soft tissue, such as spleen, kidney, liver, or brain, or from bacteria, yeast, or mammalian cells, please use our PuroSPIN™ 96 Total RNA Purification Kit (#NKP051-384). Features: - Extract total RNA from a variety of fibrous tissue samples - High throughput sample processing using 96-well silica membrane purification plates - Simple extraction process with no need for phenol or chloroform - Includes on-column/on-bead DNase I digestion step to remove contaminating DNA - High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR

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