PuroSPIN™ Fibrous Tissue RNA Purification Plus Kit, 50 Preps

PuroSPIN™ Fibrous Tissue RNA Purification Plus Kit is designed for extraction of total RNA from fibrous animal tissue, such as skin, skeletal muscle, lung, or heart. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The tissue is first disrupted through homogenization and Proteinase K treatment. The RNA is then released form the cells by the treatment with the Lysis Buffer LB-R. Following the lysis step, RNA is loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water. In comparison to our standard Total RNA Purification Kit, the Plus kit adds an additional filtration step that removes the insoluble and non-digested fraction as well as insuring elimination of genomic DNA from the lysate before loading it onto the silica spin column. This prevents contaminating materials from binding to and blocking parts of the silica membrane, leading to an increased RNA yield and higher purity of the final product. This is particularly benficial when workign with large samples that contain a lot of starting material that needs to be digested. The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN™ Fibrous Tissue RNA Purification Kitcan be used for a range of downstream applications, such as reverse-transcription PCR or qPCR, northern blotting, microarray analysis. For purification of RNA from soft tissue, such as spleen, kidney, liver, or brain, or from bacteria, yeast, or mammalian cells, please use our PuroSPIN™ Total RNA Purification Kit(#NK051-50, #NK051-250). Features: - Extract total RNA from a variety of fibrous tissue samples - Simple extraction process with no need for phenol or chloroform - Includes on-column/on-bead DNase I digestion step to remove contaminating DNA - High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR

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