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PPARγ Transcription Factor Assay Kit
品牌：Cayman
货号：10006855 规格：96 wells 目录价：￥6902
产品详情
产品名称:
PPARγ Transcription Factor Assay Kit
别名:
Peroxisome Proliferator-activated Receptor γ Transcription Factor Assay Kit
产品描述:
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.{10561} The Cayman Chemical PPARγ Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARγ binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARγ is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
保存温度:
-80
运输温度:
-80
有效期:
180
来源:
Animal/Goat|Animal/Rabbit|Animal/Bovine
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