PKCα (human, recombinant)

PKCA|PKC-A|PRKCA|Protein Kinase C Alpha Type

PKCα is an α-type conventional isoform of the PKC family of serine/threonine protein kinases, which includes the conventional isoforms PKCα, -β, and -γ, the novel isoforms PKCδ, -ε, -η, and -θ, and the atypical isoforms PKCζ and -λ/ι.{54650} PKCα is composed of an N-terminal regulatory domain containing a pseudosubstrate binding site, a C1 domain that binds the cofactor diacylglycerol (DAG), a C2 domain that binds the cofactor calcium, and a C-terminal domain containing an ATP-binding domain, substrate binding site, and catalytic domain. PKCα is ubiquitously expressed and localized to the cytoplasm in an auto-inhibited conformation until activated by calcium and DAG, which are both required for activation of the conventional PKC isoforms.{54650,54651} Activation of PKCα occurs via an allosteric and temporal mechanism during which it is translocated primarily to the plasma membrane where it phosphorylates a wide variety of substrates.{54650} PKCα is involved in the regulation of cell survival with anti- or pro-apoptotic effects and an increase or decrease in cell proliferation depending on the cellular context and cell type. It is also involved in cell differentiation and motility.{54651} Protein levels of PKCα are either increased or decreased in cancer cells, indicating a complex role in oncogenesis.{54652} Tumor levels of PKCα correlate positively to tumor grade and negatively to survival in patients with breast cancer.{54653} A SNP in PRKCA, which encodes PKCα, is associated with an increased risk of post-traumatic stress disorder (PTSD) in survivors of genocide.{54654} Cayman’s PKCα (human, recombinant) protein can be used for enzyme assay applications. This protein consists of 672 amino acids and has a calculated molecular weight of 102 kDa.

-80

-80

Lyophilized from sterile 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 0.04% Tween 20, 3 mM DTT, 20% glycerol, and 1.6 mM glutathione

180

≥80% estimated by SDS-PAGE