Indo 1-AM-细胞染料及荧光探针-深圳九游会j9网站首页有限公司

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Indo 1-AM

品牌：Adipogen

官网链接

说明书

折扣/货期

货号：CDX-I0019-M001

规格：1 mg

目录价：￥2128

产品详情

产品名称:

Indo 1-AM

别名:

Indo-1-(acetoxymethyl) ester; 4-(6-Carboxy-2-indolyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester

产品描述:

INDO 1-AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1-AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: lambdaex 330nm; lambdaem 450nm (with Calcium), lambdaex 356nm; lambdaem 478nm in methanol.

宿主来源:

Synthetic.

保存温度:

-20°C

运输温度:

Ambient

预期分子量:

1009.93

产品形式:

solid

有效期:

Stable for at least 2 years after receipt when stored at -20°C.