ImmPRESS®-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase, 15 ml

ImmPRESS AP Polymer Kit is a polymerized reporter enzyme staining system that uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The ImmPRESS™-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. Key advantages of the ImmPRESS™ Polymer System:High sensitivityLow backgroundNon-biotinMicropolymer technologyEnhanced accessibility to nuclear and membrane antigensOne-step detection with shorter assay timeReady-to-use in convenient dropper bottle with blocking solution includedSimplified multiple labelingImmunostaining ProtocolThe staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

Rat

Immunohistochemistry / Immunocytochemistry,In situ hybridization,ELISAs

Micropolymer AP

2-8 °C

Ambient

Ready-to-Use

美国