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FURA-2 pentapotassium salt
品牌：Adipogen
货号：CDX-F0013-M001 规格：1 mg 目录价：￥1498
产品详情
产品名称:
FURA-2 pentapotassium salt
别名:
Fura-2 . 5K
产品描述:
Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.
宿主来源:
Synthetic
保存温度:
-20°C
运输温度:
BI
预期分子量:
831.99
产品形式:
solid
有效期:
Stable for at least 2 years after receipt when stored at -20°C.
产地:
瑞士
纯度:
>90% (HPLC)
CAS号:
113694-64-7
分子式:
C29H22N3O14 . 5K
溶解度:
Soluble in water or DMSO.
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