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DAN

品牌：Adipogen

官网链接

说明书

折扣/货期

货号：CDX-D0062-G001	规格：1 g	目录价：￥2156
货号：CDX-D0062-G005	规格：5 g	目录价：￥7378

产品详情

产品名称:

DAN

别名:

2,3-Diaminonaphthalene

产品描述:

The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.

宿主来源:

Synthetic.

保存温度:

+4°C

运输温度:

Ambient

预期分子量:

158.2

产品形式:

solid

有效期:

Stable for at least 2 years after receipt when stored at +4°C.