ThelioKeep是一种保存液，适用于皮肤和神经组织保存在4℃。该保存液不含DMSO、血清、蛋白质和外来成分。ThelioKeep能保持这些组织的结构强度和活性。 

产品特点： 

● ThelioKeep在4℃下保存组织可保存1 - 2周 

● 维持上皮、内皮和神经组织的形态和增殖能力。对于外周神经，也可以维持电生理功能 

● ThelioKeep中的茶多酚（(EGCG ; Epigallocatechin gallate）可以保护薄而脆弱的组织 

● 与多种哺乳动物样本兼容。 

● 保存在ThelioKeep中的组织可用于各种用途 

● ThelioKeep中没有血清，没有DMSO，没有蛋白质 

● 保证高的细胞活力，无DMSO细胞毒性或血清衍生蛋白污染的风险 

● 没有细菌、真菌和支原体污染 

● 保质期长，ThelioKeep在4℃环境能稳定保存1年时间 

● 成分是： Inorganic ions, D-Glucose, Amino Acid, pH adjuster, Epigallocatechin gallate和Phenol Red 

ThelioKeep使用步骤： 

1. ThelioKeep-EGCG的制备:从瓶中取出0.5 ~ 1.0ml ThelioKeep，在无菌条件下倒入装有EGCG片剂的管中，将片剂完全溶解在管中。用移液管将全部溶液移回至ThelioKeep瓶中，并充分混合。 

2. 取出适量准备好的ThelioKeep-EGCG至干净的试管中，然后冷却至4~10℃ 

3. 将从实验动物上分离出的上皮或者内皮组织浸入到冷却的ThelioKeep-EGCG中，盖好盖子保存在4℃冰箱1~2周 

恢复步骤： 

1. 在冷的PBS中轻轻清洗保存的组织几次。将组织转移到实验介质中。 

2. 如果是活体组织用于实验或培养时，尽快将其置于生理条件下。 

3. 如果是制备载玻片标本时，从显微镜下取出组织后，立即将组织转移到固定剂中。 

4. 当从保存的细胞中提取细胞质物质时，请用冷的PBS充分洗涤细胞。 

产品使用实例： 

 Human corneal endothelium tissue were preserved for 2 weeks at 4℃.
(1) Left : When using another supplier's tissue culture medium, 
cell morphology was completely disappeared.
(1) Right: When using ThelioKeep, hexagonal shape of the 
cytoplasmic membrane was well maintained.
(2) When ThelioKeepwas used, the cell proliferation speed was 
about twice compared to when another supplier's tissue 
culture medium was used. 

 Rat peripheral nerve tissue preserved for 2 weeks at 4℃were transplanted to another rat and isolated after 24 hours. 
(3) Left : When using another supplier's tissue culture medium, 
structure was completely disrupted.
(3) Right: When using ThelioKeep, structure was well maintained.
(4) When ThelioKeepwas used, electric conductivity was also
maintained as well as fresh tissue.

产品订购详情： 

更多产品详情请联系Funakoshi中国授权代理商欣博盛生物 

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