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折扣/货期| 货号：CDX-C0011-M100 | 规格：100 mg | 目录价：￥1680 |
产品详情
产品名称:
5(6)-FAM N-succinimidyl ester
别名:
5(6)-Carboxyfluorescein NHS ester (mixture of isomers); 5(6)-CFSE; 5(6)-FAM SE; FLUOS
产品描述:
Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.
宿主来源:
Synthetic.
保存温度:
-20°C
运输温度:
Ambient
预期分子量:
473.4
产品形式:
solid
有效期:
Stable for at least 2 years after receipt when stored at -20°C.
产地:
瑞士
纯度:
>98% (HPLC)
CAS号:
117548-22-8
分子式:
C25H15NO9
溶解度:
Soluble in DMSO or dimethylformamide.
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