5' EndTag™ DNA/RNA Labeling Kit, 1 Kit

5' EndTag Labeling DNA/RNA Kit Enables simple and uniform labeling of 5' ends of the DNA, RNA or unmodified oligonucleotides. It is ideal for labeling of PCR primers beacuse it leaves 3' end unmodified.Choice of diverse labels - fluorophores, biotin, haptens, affinity ligands (not included) Kit components:The kit is designed to perform 10 end labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 ug of a 25 base oligo) per reaction and includes:T4 polynucleotide kinase10x reaction bufferATPγSPrecipitantAlkaline Phosphatase Note: Requires thiol-reactive labeling reagent:Biotin (Long Arm) Maleimide DNP MaleimideFluorescein (Long Arm) MaleimideFucose MaleimideTexas Red® MaleimideNote on Shipping: This item is shipped on ice requiring additional shipping charges. End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding.5' or 3' EndTag™ labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.Both the 5' EndTag™ and the 3' EndTag™ Nucleic Acid Labeling Systems enable the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of the nucleic acids using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.Labeling time is about 1 hour with very little hands-on time.5' EndTag™ Labeling KitThe 5' EndTag™ System labels 5' ends of DNA, RNA, or unmodified oligonucleotides. 5' EndTag™ is ideal for labeling PCR primers because a label is attached only at the 5' end, leaving the 3' end available for polymerization.Labeling with the 5' EndTag™ Kit is achieved in two steps:1. T4 polynucleotide kinase transfers a thiophosphate from ATPγS to the 5' -OH group of the nucleic acid. (5' phosphorylated ends are converted to 5' -OH groups with the included alkaline phosphatase).2. Thiolated sample is coupled to a thiol-reactive label. The 5' EndTag™ Kit is designed to perform 10 labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 ug of a 25 base oligo) per reaction. Thiol-reactive label is not included in the kit and should be selected separately."

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