研发背景：
西尼罗河病毒(WNV)是一种由蚊虫类介导传播的黄病毒，与引起人类感染性流行病的登革热病毒、黄热病病毒和日本脑炎病毒密切相关。
WNV和登革热病毒(DENV)同属黄病毒科（Flaviviridae）黄热病毒属，是具有小包膜单链RNA的病毒。黄热病病毒的病毒包膜由多拷贝的50-60kDa E蛋白和8kDa M（膜）蛋白组成。X射线晶体学研究表明，每种E蛋白的胞外域由三个结构域组成：DI，DII和DIII，三者通过柔性铰链连接。DII结构域的尖端包含“融合环”保守区域，是病毒包膜与宿主细胞膜膜融合所必需的区域。
E60单克隆抗体可与WNV和DENV病毒包膜E蛋白反应。靶向DIII表位的疫苗策略可引发针对黄病毒病的安全有效的免疫应答。此抗体也将成为WNV和DENV病毒研究的一个有力工具。
产品信息：
产品货号 | BE0325 |
产品名称 | InVivoMAb anti-West Nile/dengue virus E protein |
规格 | 1mg,5mg,25mg, 50mg, 100mg |
克隆号 | E60 |
同种型(Isotype) | Mouse IgG2a, κ |
免疫原(Immunogen) | Purified soluble WNV E protein |
成分(Formulation) | PBS, pH 7.0 |
Contains no stabilizers or preservatives |
内毒素(Endotoxin) | <2EU/mg (<0.002EU/μg) |
Determined by LAL gel clotting assay |
纯度(Purity) | >95% |
Determined by SDS-PAGE |
无菌(Sterility) | 0.2 μM filtered |
生产(Production) | Purified from tissue culture supernatant in an animal free facility |
纯化(Purification) | Protein A |
保存(Storage) | The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
应用(Reported Applications) | Neutralization of WNV |
Neutralization of DENV1,2,3,4 |
Application References:
Goo, L., et al. (2018). 'The Zika virus envelope protein glycan loop regulates virion antigenicity.' Virology 515: 191-202.
Dowd, K. A., et al. (2011). 'A dynamic landscape for antibody binding modulates antibody-mediated neutralization of West Nile virus.' PLoS Pathog 7(6): e1002111.
Oliphant, T., et al. (2006). 'Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein.' J Virol 80(24): 12149-12159.
Nybakken, G. E., et al. (2005). 'Structural basis of West Nile virus neutralization by a therapeutic antibody.' Nature 437(7059): 764-769.
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