• 九游会j9网站首页,j9九游会首页登录,j9九游会ag真人官网

    您好,欢迎光临九游会j9网站首页商城!
    服务热线:400 680 0892
    新闻资讯 News

    Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

    2023-10-26
    浏览次数: 48

    EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher生产的在E. coli中表达的重组人单核小体(组蛋白H2A、H2B、H3和H4各2个;accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4-P62805)由601位处的147个DNA碱基对缠绕在组蛋白上构成。组蛋白H2B(由专门的半合成方法产生)在120位处含有泛素赖氨酸。核小体是染色质的基本单位,Lowary和Widom鉴定的601序列是一个含有147碱基对的序列,对组蛋白八聚体具有高亲和力,可用于核小体组装,并含有5'biotin-TEG基团。适合用作酶筛选分析或效应蛋白结合实验的底物。

    [1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715


    产品详情

    标签: Biotinylated

    保存温度:Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

    产品形式: H2BK120ub dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 22.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.12 μM. MW = 216,985.9 Da.


    验证数据

    Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

    Figure 1: Western blot data

    Western Analysis of Nucleosome, Recombinant Human, H2BK120ub. 

    Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes 

    (Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL

     readout. Only the H2BK120ub sample produced a detectable signal. Bottom 

    Panel: Detail from Coomassie stained gel showing unmodified nucleosomes 

    (Lane 1) and H2BK120ub nucleosomes (Lane 2).

    Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

    Figure 2: Mass spec data H2BK120ub protein analyzed by high 

    resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined 

    mass = 22,320.3 Da.

    Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

    Figure 3: Protein gel data

    Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant 

    Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the 

    preparation. Sizes of molecular weight markers and positions of the core 

    histones (H2A, H2BK120ub, H3.1, and H4) are indicated.

    Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

    Figure 4: DNA gel data

    Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native 

    PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free 

    DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng).


    订购详情

    货号

    产品名称

    规格

    16-0396   

    Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated   

    25 µg   



    Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

    如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物 

    全国服务热线: 4006-800-892       邮箱: market@qckc0531.com 

    深圳: 0755-26755892         北京: 010-88594029            

    广州:020-87615159           上海: 021-34613729

    代理品牌网站: www.nobiosescience.com 

    自主品牌网站: www.neobiosescience.net

    分享到:
    关注欣博盛公众号
    Copyright ©2021 - 2023 深圳九游会j9网站首页有限公司
      网站地图
    犀牛云提供企业云服务
    九游会j9网站首页