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Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein, 5 mg/刺桐凝集素，FLUORESCEIN标记
品牌： Vectorlabs
产品货号： FL-1141-5
规格： 5 mg

报价：
折后价：

数量： - +
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
  • 产品名称:
    Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein, 5 mg/刺桐凝集素，FLUORESCEIN标记
  • 别名:
    刺桐凝集素，FLUORESCEIN标记
  • 产品描述:
    Erythrina cristagalli lectin ECL, ECA consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins.This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.Fluorescein labeled Erythrina cristagalli lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.Inhibiting/Eluting Sugar: 200 mM lactose
  • 反应种属:
  • 宿主来源:
  • 实验应用:
    Immunofluorescence,Glycobiology
  • 靶标/特异性:
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  • 推荐稀释度:
    The recommended concentration range for use is 5-20µg/ml.
  • 免疫原:
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  • 偶联:
    Fluorescein
  • 产品浓度:
    5 mg active conjugate/ml
  • 表达系统:
  • 阳性对照:
  • 保存温度:
    2° - 8°C – Do Not Freeze
  • 运输温度:
    Ambient
  • 细胞定位:
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  • 产品形式:
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
  • 有效期:
  • 产地:
    美国
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  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
  • 产品特点:
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