Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)和NUCLEAR-ID ® Red DNA Stain是细胞渗透性染料，适用于活细胞的细胞核染色。这系列染料为通过流式细胞术研究细胞周期进程的诱导和抑制提供了一种简便方法。该试剂在活细胞研究中的潜在应用包括确定细胞DNA含量和细胞周期分布、检测生长模式的变化、监测细胞凋亡以及评估肿瘤细胞行为和抑制基因机制。
NUCLEAR-ID® Red DNA stain
产品特点
● 远红色荧光特异性DNA染料
● 稳定且纯度高
● 可分析活细胞、可渗透细胞和固定细胞
● 无光漂白作用
● 无需RNase处理
● 与GFP和FITC兼容
● 不需要紫外线激光源激发
● 经验证适用于各种细胞密度
● 快速且易于使用
实例分析
在37°C下用终浓度为4000x或2000x的NUCLEAR-ID ® Red DNA Stain或同等µM浓度的竞争品牌染料对细胞进行染色，并在染色后轻轻洗涤。在15分钟和24小时对细胞成像，结果显示NUCLEAR-ID ® Red DNA Stain在更低的浓度就能显现dsDNA。
产品详情：
产品名称 | NUCLEAR-ID® Red DNA stain |
产品货号 | ENZ-52406 |
产品规格 | 200µl |
最大激发波长 | 566nm |
最大发射波长 | 650nm |
纯度 | ≥93%（HPLC） |
质量控制 | 1.Absorption peak of NUCLEAR-ID® Red dye: λmax = 566 ± 4 nm 2.% purity of NUCLEAR-ID® Red dye by HPLC: ≥93% |
应用 | Flow Cytometry, Fluorescent detection |
短期储存 | -20°C |
长期储存 | -80°C |
产品说明 | The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
部分产品文献引用
1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)
2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)
3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)
4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)
5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)
6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)
7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)
NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)
产品特点
● 蓝色荧光特异性DNA染料
● 稳定且纯度高
● 可分析活细胞、可渗透细胞和固定细胞
● 无光漂白作用
● 无需RNase处理
● 与GFP和红色染料兼容
● 经验证适用于各种细胞密度
● 快速易用
产品详情：
产品名称 | NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®) |
产品货号 | ENZ-CHM103-0200 |
产品规格 | 200µl |
最大激发波长 | 350nm |
最大发射波长 | 461nm |
纯度 | ≥95% (HPLC) |
外观 | Frozen liquid |
应用 | Flow Cytometry, Fluorescent detection |
短期储存 | -20°C, Protect from light. Avoid freeze/thaw cycles |
长期储存 | -80°C, Protect from light. Avoid freeze/thaw cycles |
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